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VLDLR Antibody (6A6) - BSA Free

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Western Blot: VLDLR Antibody (6A6) [NBP1-78162] - MCF-7 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. VLDLR Antibody (NBP1-78162) antibody at 1:1000, 4C, ...read more
Immunocytochemistry/ Immunofluorescence: VLDLR Antibody (6A6) [NBP1-78162] - VLDL R Antibody (6A6) [NBP1-78162] - VLDL Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained ...read more
Immunohistochemistry: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor in mouse kidney.
Western Blot: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor expression in mouse kidney.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
6A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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VLDLR Antibody (6A6) - BSA Free Summary

Immunogen
Synthetic peptide corresponding to the C-terminus of human VLDL Receptor [Swiss-Prot# P98155]
Localization
Membrane; Single-pass type I membrane protein. Membrane > clathrin-coated pit; Single-pass type I membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VLDLR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000
Application Notes
This VLDL Receptor (6A6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where various isoforms can be detected between 120 and 168 kDa (see reference Jokinen et al, 1994).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-78162 in the following applications:

Publications
Read Publications using
NBP1-78162 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for VLDLR Antibody (6A6) - BSA Free

  • CARMQ1
  • CHRMQ1
  • FLJ35024
  • very low density lipoprotein receptor
  • very low-density lipoprotein receptor
  • VLDL R
  • VLDL receptor
  • VLDLR
  • VLDL-R
  • VLDLRCH

Background

VLDL (very low density lipoprotein) cholesterol is one of the three major types of cholesterol found in blood. VLDL receptor plays an essential role in triglyceride metabolism. It binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VLDLR Antibody (NBP1-78162)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(5)
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Human
(2)
Mouse
(2)
Rat
(1)
Bovine
(1)
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Showing Publications 1 - 8 of 8.
Publications using NBP1-78162 Applications Species
Qu F, Brough SC, Michno W et al. Crosstalk between small-cell lung cancer cells and astrocytes mimics brain development to promote brain metastasis Nature cell biology 2023-10-01 [PMID: 37783795]
Oshio Y, Hattori Y, Kamata H et al. Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice Scientific reports 2021-04-13 [PMID: 33850206] (WB, Mouse) WB Mouse
Velagapudi S, Schraml P et al. Scavenger receptor BI promotes cytoplasmic accumulation of lipoproteins in clear-cell renal cell carcinoma. J Lipid Res 2018-01-11 [PMID: 30173145] (WB, Human) WB Human
Lee HC, Lin HT, Ke LY et al. VLDL from Metabolic Syndrome Individuals Enhanced Lipid Accumulation in Atria with Association of Susceptibility to Atrial Fibrillation. Int J Mol Sci. 2016-01-25 [PMID: 26805814] (ICC/IF, Mouse) ICC/IF Mouse
Niemeier A, Kassem M, Toedter K, Wendt D, Ruether W, Beisiegel U, Heeren J. Expression of LRP1 by human osteoblasts: a mechanism for the delivery of lipoproteins and vitamin K1 to bone. J Bone Miner Res. 20(2):283-93. 2005-02-01 [PMID: 15647823] (WB, Human) WB Human
Roberts CK, Barnard RJ, Liang KH, Vaziri ND. Effect of diet on adipose tissue and skeletal muscle VLDL receptor and LPL: implications for obesity and hyperlipidemia. Atherosclerosis. 161(1):133-41. 2002-03-01 [PMID: 11882325]
Wyne KL, Pathak K, Seabra MC, Hobbs HH. Expression of the VLDL receptor in endothelial cells. Arterioscler Thromb Vasc Biol. 16(3):407-15. 1996-03-01 [PMID: 8630667] (WB, Bovine) WB Bovine
Jokinen EV, Landschulz KT, Wyne KL, Ho YK, Frykman PK, Hobbs HH. Regulation of the very low density lipoprotein receptor by thyroid hormone in rat skeletal muscle. J Biol Chem. 269(42):26411-8. 1994-10-21 [PMID: 7929362] (WB, Rat) WB Rat

Review for VLDLR Antibody (NBP1-78162) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-78162:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot VLDLR NBP1-78162
Enlarge
5
reviewed by:
Verified Customer
WB Human 11/21/2020
View

Summary

ApplicationWestern Blot
Sample Testedbreast cancer MCF7 cells
SpeciesHuman
LotB-4

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VLDLR Antibody (NBP1-78162). (Showing 1 - 1 of 1 FAQs).

  1. Does VLDL Receptor Antibody (6A6) (NBP1-78162) cross react with Rhesus tissue for IHC?
    • Our VLDL receptor antibody has not yet been tested in Macaca mulatta (Rhesus macaque). Based on the sequence homology, it looks like there is a good chance that it will detect the Rhesus protein. If you would like to try this antibody in IHC with a Rhesus sample, and if you can submit a review of the product afterwards, you can take advantage of our Innovator's Reward program. In exchange for your review of our product in an untested species or application, we will issue you a credit for the purchase price of the antibody. Contact us at innovators@novusbio.com for more information.
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Recent Reviews

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5
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Verified Customer
11/21/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol VLDLR
Entrez
Uniprot