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Vinculin Antibody [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-41274AF350
Alexa Fluor 405
NBP2-41274AF405
Alexa Fluor 488
NBP2-41274AF488
Alexa Fluor 532
NBP2-41274AF532
Alexa Fluor 594
NBP2-41274AF594
Alexa Fluor 647
NBP2-41274AF647
Alexa Fluor 700
NBP2-41274AF700
Alexa Fluor 750
NBP2-41274AF750
Biotin
NBP2-41274B
CoraFluor 1
NBP2-41274CL1
DyLight 350
NBP2-41274UV
DyLight 405
NBP2-41274V
DyLight 488
NBP2-41274G
DyLight 550
NBP2-41274R
DyLight 594
NBP2-41274DL594
DyLight 650
NBP2-41274C
DyLight 680
NBP2-41274FR
DyLight 755
NBP2-41274IR
FITC
NBP2-41274F
HRP
NBP2-41274H
Janelia Fluor 525
NBP2-41274JF525
Janelia Fluor 646
NBP2-41274JF646
Janelia Fluor 669
NBP2-41274JF669
PerCP
NBP2-41274PCP
Unconjugated
NBP2-41274
mFluor Violet 450 SE
NBP2-41274MFV450
mFluor Violet 500 SE
NBP2-41274MFV500
mFluor Violet 610 SE
NBP2-41274MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-41274
BSA Free
NBP2-41274
BSA Free
NBP2-41274-0.025mg
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Vinculin Antibody [Janelia Fluor® 549] Summary

Immunogen
Antibody was raised against a peptide corresponding to 23 amino acids near the center of human Vinculin. < Amino Acid Sequence: VREAFQPQEPDFPPPPPDLEQLR
Specificity
Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist. Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD). Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD). NBP2-41274 can detect human, mouse and rat.
Predicted Species
Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VCL
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Vinculin Antibody [Janelia Fluor® 549]

  • CMD1W
  • CMH15
  • metavinculin
  • MVCL
  • VCL
  • Vinculin

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Vinculin Antibody (NBP2-41274JF549). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VCL