Vimentin Antibody (V9) - BSA Free

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 1 application: ICC/IF.

Showing Publications 1 - 4 of 4.

Publications using NBP1-97670	Applications	Species
Tamai K, Sakai K, Yamaki H et al. iPSC-derived mesenchymal cells that support alveolar organoid development Cell Reports Methods 2022-10-24 [PMID: 36313800]
Xie J, Yuan S, Peng L et al. Antitumor immunity targeting fibroblast activation protein‑ alpha in a mouse Lewis lung carcinoma model Oncol Lett 2020-05-18 [PMID: 32566014]
Rigoutsos I, Lee SK, Nam SY et al. N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration. Genome Biol. 2017-05-24 [PMID: 28535802]
Hu R, Ling W, Xu W, Han D. Fibroblast-like cells differentiated from adipose-derived mesenchymal stem cells for vocal fold wound healing. PLoS ONE 2014-03-25 [PMID: 24664167] (ICC/IF, Canine)	ICC/IF	Canine

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-P	Human	06/05/2023	View Summary Application Immunohistochemistry-Paraffin Sample Tested Tonsil Species Human Lot 18495
	5 reviewed by: Verified Customer	IHC-P	Equine	06/05/2023	View Summary Application Immunohistochemistry-Paraffin Sample Tested Intestine Species Equine Lot 18495 Comments Comments ***Novus Innovators Program - new species or application used on a primary antibody.***
	5 reviewed by: Verified Customer	IHC-P	Feline	06/05/2023	View Summary Application Immunohistochemistry-Paraffin Sample Tested Intestine Species Feline Lot 18495 Comments Comments ***Novus Innovators Program - new species or application used on a primary antibody.*** Requires heat-induced epitope retrieval. I used Target Retrieval Solution heated to 95 degrees Celsius using a vegetable steamer. Slides were left in pre-heated solution for 20m.
	5 reviewed by: Verified Customer	IHC-P	Canine	06/05/2023	View Summary Application Immunohistochemistry-Paraffin Sample Tested Intestine Species Canine Lot 18495

Array NBP1-97670

• Vimentin Antibodies
• Vimentin ELISA Kits
• Vimentin Lysate
• Vimentin Proteins
• Vimentin Proteins and Enzyme