![Western Blot: Versican Antibody (S351-23) [NBP2-22408] - Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versicanprotein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (NBP2-22408). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (NBP2-22408) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.](http://images.novusbio.com/fullsize/Versican-Antibody-S351-23-Western-Blot-NBP2-22408-img0004.jpg "Western Blot: Versican Antibody (S351-23) [NBP2-22408] - Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versicanprotein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (NBP2-22408). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (NBP2-22408) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | S351-23 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein |
| Localization | Extracellular Space , Extracellular Matrix |
| Specificity | Detects >350kDa. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | VCAN |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of Versican Antibody was sufficient for detection of Versican in 20 ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Publication using NBP2-22408 | Applications | Species |
|---|---|---|
| Liu Z, Huang J, Kang D et al. Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering Acta biomaterialia 2022-11-05 [PMID: 36347448] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Versican Antibody (NBP2-22408)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VersicanAntibody (D) Composite.](http://images.novusbio.com/fullsize/Versican-Antibody-S351-23-Immunocytochemistry-Immunofluorescence-NBP2-22408-img0005.jpg "Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VersicanAntibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Versican Antibody (D) Composite.](http://images.novusbio.com/fullsize/Versican-Antibody-S351-23-Immunocytochemistry-Immunofluorescence-NBP2-22408-img0003.jpg "Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Versican Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VersicanAntibody. (D) Composite.](http://images.novusbio.com/fullsize/Versican-Antibody-S351-23-Immunocytochemistry-Immunofluorescence-NBP2-22408-img0006.jpg "Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VersicanAntibody. (D) Composite.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")