Home » VEGFR3/Flt-4 » VEGFR3/Flt-4 Antibodies » VEGFR3/Flt-4 Antibody (AFL4) - BSA Free
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VEGFR3/Flt-4 Antibody (AFL4) - BSA Free

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Immunohistochemistry-Frozen: VEGFR3/Flt-4 Antibody (AFL4) [NBP1-43259] - VEGFR-3 (green) was detected in murine cancer using VEGFR-3-AlexaFluor488 antibody (1:50; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review using the Alexa Fluor488 version of this VEGFR-3 antibody.
Immunohistochemistry-Frozen: VEGFR3/Flt-4 Antibody (AFL4) [NBP1-43259] - VEGFR-3 (green) was detected in murine cancer using VEGFR-3-AlexaFluor488 antibody (1:50; 1 hour) in PBS. Nuclei were stained with DAPI (blue). ...read more
Flow Cytometry: VEGFR3/Flt-4 Antibody (AFL4) [NBP1-43259] - Surface staining of MC/9 cell line with Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (left) and PE (right). Appropriate isotype controls were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, Dual ISH-IHC
Clone
AFL4
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-43259AF405
Alexa Fluor 488
NBP1-43259AF488
Alexa Fluor 647
NBP1-43259AF647
Alexa Fluor 700
NBP1-43259AF700
Allophycocyanin
NBP1-43259APC
Allophycocyanin/Cy7
NBP1-43259APCCY7
Biotin
NBP1-43259B
Biotin
NBP1-43502-0.1mg
DyLight 350
NBP1-43259UV
DyLight 405
NBP1-43259V
DyLight 488
NBP1-43259G
DyLight 755
NBP1-43259IR
FITC
NBP1-43259F
HRP
NBP1-43259H
PE
NBP1-43259PE
PE/Cy7
NBP1-43259PECY7
PerCP
NBP1-43259PCP
mFluor Violet 450 SE
NBP1-43259MFV450
mFluor Violet 500 SE
NBP1-43259MFV500
mFluor Violet 610 SE
NBP1-43259MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-43259-0.5mg
BSA Free
NBP1-43259-0.05mg
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VEGFR3/Flt-4 Antibody (AFL4) - BSA Free Summary

Immunogen
The immunogen for this antibody was VEGFR3.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
FLT4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. Dual ISH-IHC validation (PMID: 31083674).
Publications
Read Publications using
NBP1-43259 in the following applications:

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for VEGFR3/Flt-4 Antibody (AFL4) - BSA Free

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195 kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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VEGFR3/Flt-4
Antibody (AFL4) - BSA
NBP1-43259
VEGFR3/Flt-4 Antibody (AFL4) - BSA Free
Species: Mu
Applications: WB, Flow, IHC, IP, Dual ISH-IHC

Publications for VEGFR3/Flt-4 Antibody (NBP1-43259)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Dual ISH-IHC, FLOW.


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Dual ISH-IHC
(1)
FLOW
(1)
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Mouse
(2)
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Reviews for VEGFR3/Flt-4 Antibody (NBP1-43259) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FLT4