Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Human VDR/NR1I1/Vitamin D Receptor in samples. No Significant cross-reactivity or interference between Human VDR/NR1I1/Vitamin D Receptor and analogues was observed. |
Standard Curve Range |
0.63 - 40 ng/mL |
Sensitivity |
0.38 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 5.27 |
Intra-Assay |
CV% < 5 |
Spike Recovery |
85-102% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
VDR |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric)
Background
Vitamin D Receptor encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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