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Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric)

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ELISA: Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) [NBP2-82389] - Samples were spiked with high concentrations of Human VDR/NR1I1/Vitamin D Receptor and diluted with Reference Standard & Sample Diluent ...read more
ELISA: Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) [NBP2-82389] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

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Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human VDR/NR1I1/Vitamin D Receptor in samples. No Significant cross-reactivity or interference between Human VDR/NR1I1/Vitamin D Receptor and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.27
Intra-Assay
CV% < 5
Spike Recovery
85-102%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
VDR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric)

  • NR1I1
  • NR1I1Nuclear receptor subfamily 1 group I member 11,25-dihydroxyvitamin D3 receptor
  • VDR
  • vitamin D1,25- dihydroxyvitamin D3 receptor
  • vitamin D3 receptor

Background

Vitamin D Receptor encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol VDR