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VDAC1 Antibody (S152B-23)

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Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187] - Western Blot analysis of Mouse Brain and Human RT-4 lysates showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (NBP2-42187). Lane 1: Molecular Weight Ladder. Lane A: Mouse Brain Lysate. Lane B: Human RT-4 Lysate. Block: 5% Skim milk in TBST . Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (NBP2-42187) at 1:200 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: ~30 kDa.
Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187] - Western Blot analysis of Mouse Brain and Human RT-4 lysates showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 ...read more
Immunocytochemistry/ Immunofluorescence: VDAC1 Antibody (S152B-23) [NBP2-42187] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VDAC1 ...read more
Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (NBP2-42187). Lane ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
S152B-23
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-42187AF405
Alexa Fluor 488
NBP2-42187AF488
Alexa Fluor 647
NBP2-42187AF647
Alexa Fluor 700
NBP2-42187AF700
Biotin
NBP2-42187B
DyLight 350
NBP2-42187UV
DyLight 405
NBP2-42187V
DyLight 488
NBP2-42187G
DyLight 550
NBP2-42187R
DyLight 650
NBP2-42187C
DyLight 680
NBP2-42187FR
DyLight 755
NBP2-42187IR
FITC
NBP2-42187F
HRP
NBP2-42187H
Janelia Fluor 549
NBP2-42187JF549
Janelia Fluor 646
NBP2-42187JF646
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VDAC1 Antibody (S152B-23) Summary

Immunogen
Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3.
Localization
Mitochondrion , Mitochondrion Outer Membrane , Cell Membrane
Specificity
Greater then 60% identity with VDAC2 and VDAC3.Detects approx 30kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
VDAC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:1000
Application Notes
1 ug/ml of VDAC1 Antibody was sufficient for detection of VDAC1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for VDAC1 Antibody (S152B-23)

  • hVDAC1
  • MGC111064
  • Outer mitochondrial membrane protein porin 1
  • Plasmalemmal porin
  • Porin 31HL
  • Porin 31HM
  • PORIN
  • PORIN-31-HL
  • VDAC
  • VDAC1
  • VDAC-1
  • voltage-dependent anion channel 1
  • voltage-dependent anion-selective channel protein 1

Background

VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VDAC1
Uniprot