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VASP [p Ser155] Antibody - Azide and BSA Free

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Western Blot: VASP [p Ser155] Antibody [NBP3-05448] - Western blot analysis of extracts of A431 cells, using VASP antibody (NBP3-05448) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes ...read more
Immunohistochemistry-Paraffin: VASP [p Ser155] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded mouse lung using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: VASP [p Ser155] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded rat brain using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: VASP [p Ser155] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded human colon using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).
Immunoprecipitation: VASP [p Ser155] Antibody [NBP3-05448] - Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug VASP pAb (NBP3-05448). Western blot was performed from the immunoprecipitate using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

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VASP [p Ser155] Antibody - Azide and BSA Free Summary

Immunogen
A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1). RVSNA
Modification
p Ser155
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VASP
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:50 - 1:100
  • Western Blot 1:500 - 1:2000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for VASP [p Ser155] Antibody - Azide and BSA Free

  • vasodilator-stimulated phosphoprotein
  • VASP

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VASP