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VASP [p Ser238] Antibody

Images

 
Western Blot: VASP [p Ser238] Antibody [NB100-82254] - Analysis of extracts from serum-treated 293 and PMA-treated C6 cells using VASP (Phospho-Ser239) Antibody
Immunohistochemistry-Paraffin: VASP [p Ser238] Antibody [NB100-82254] - Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue, using NB100-82254 (left) or the same antibody preincubated with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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VASP [p Ser238] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 238 (K-V-SP-K-Q).
Modification
p Ser238
Marker
Focal Adhesion Marker
Specificity
Detects endogenous levels of VASP only when phosphorylated at serine 238.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VASP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
VASP Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VASP [p Ser238] Antibody

  • vasodilator-stimulated phosphoprotein
  • VASP

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for VASP Antibody (NB100-82254) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VASP
Entrez
Uniprot