![Western Blot: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-S15-4-Western-Blot-NBP2-12909-img0008.jpg "Western Blot: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, MA |
| Clone | S15/4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide amino acids 774-791 (C-terminus) of rat TrpV3 |
| Localization | Membrane |
| Specificity | Detects approx 70 kDa. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TRPV3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of TrpV3 Antibody was sufficient for detection of TrpV3 in 10 ug of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Vanilloid R-like 3/TRPV3 Antibody (NBP2-12909)Find related products by research area.
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| Gene Symbol | TRPV3 |
![Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-S15-4-Immunocytochemistry-Immunofluorescence-NBP2-12909-img0011.jpg "Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.")
![Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-S15-4-Immunohistochemistry-NBP2-12909-img0010.jpg "Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.")
![Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-S15-4-Immunohistochemistry-NBP2-12909-img0009.jpg "Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.")
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