![Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Analysis of canine bladder tissue using Uroplakin Ia antibody. Uroplakin Ia antibody used at 1:500 concentration in 3% BSA and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in Tris/EDTA buffer, pH 9. Image from verified customer review.](http://images.novusbio.com/fullsize/Uroplakin-Ia-Antibody-UPK1A-2921-Immunohistochemistry-Paraffin-NBP2-79733-img0005.jpg "Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Analysis of canine bladder tissue using Uroplakin Ia antibody. Uroplakin Ia antibody used at 1:500 concentration in 3% BSA and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in Tris/EDTA buffer, pH 9. Image from verified customer review.")
| Reactivity | Hu, CaSpecies Glossary |
| Applications | ELISA, IHC, MA |
| Clone | UPK1A/2921 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79868) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa 114-173) of human Uroplakin Ia (UPK1A) protein (exact sequence is proprietary) (Uniprot: O00322) |
| Localization | Cell Surface |
| Marker | Urothelial Differentiation Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | UPK1A |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: For coating, order Ab without BSA. Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 29 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
Affinity Purification | Canine | 03/08/2022 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Uroplakin Ia Antibody (NBP2-79733)Find related products by research area.
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| Verified Customer 03/08/2022 |
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| Application: | Affinity Purification | |
| Species: | Canine |
![Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin Ia Antibody (UPK1A/2921).](http://images.novusbio.com/fullsize/Uroplakin-Ia-Antibody-UPK1A-2921-Immunohistochemistry-Paraffin-NBP2-79733-img0001.jpg "Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin Ia Antibody (UPK1A/2921).")
![Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urinary Bladder stained with Uroplakin Ia Antibody (UPK1A/2921).](http://images.novusbio.com/fullsize/Uroplakin-Ia-Antibody-UPK1A-2921-Immunohistochemistry-Paraffin-NBP2-79733-img0002.jpg "Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urinary Bladder stained with Uroplakin Ia Antibody (UPK1A/2921).")
![Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin Ia Antibody (UPK1A/2921).](http://images.novusbio.com/fullsize/Uroplakin-Ia-Antibody-UPK1A-2921-Immunohistochemistry-Paraffin-NBP2-79733-img0003.jpg "Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin Ia Antibody (UPK1A/2921).")
![Protein Array: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Uroplakin Ia Antibody (UPK1A/2921) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Uroplakin-Ia-Antibody-UPK1A-2921-Protein-Array-NBP2-79733-img0004.jpg "Protein Array: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Uroplakin Ia Antibody (UPK1A/2921) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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