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Uromodulin Antibody (774056)

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Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more
Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
774056
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5175-SP
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Uromodulin Antibody (774056) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Uromodulin
Ser24-Ala618
Accession # Q91X17
Specificity
Detects mouse Uromodulin in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human Uromodulin is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
UMOD
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB5175 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Uromodulin Antibody (774056)

  • ADMCKD2
  • FJHN
  • HNFJ
  • HNFJ1
  • MCKD2
  • Tamm-Horsfall glycoprotein
  • Tamm-Horsfall urinary glycoprotein
  • THGP
  • THP
  • UMOD
  • uromodulin (uromucoid, Tamm-Horsfall glycoprotein)
  • Uromodulin
  • Uromucoid

Background

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105‑120 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑148), a ZP domain that mediates oligomerization (aa 335‑590), and a cleavable C-terminal propeptide (aa 619‑642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17 aa substitution for aa 601‑642, and a 166 aa substitution for aa 441‑642. Over aa 25‑618, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Uromodulin Antibody (MAB5175)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Western Blot.


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IHC
(2)
IHC-Fr
(2)
IHC-P
(1)
Western Blot
(1)
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Mouse
(6)
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Showing Publications 1 - 8 of 8.
Publications using MAB5175 Applications Species
S Kumar, X Fan, H Milo Rasou, R Sharma, DJ Salant, W Lu ZEB2 controls kidney stromal progenitor differentiation and inhibits abnormal myofibroblast expansion and kidney fibrosis JCI Insight, 2023-01-10;0(0):. 2023-01-10 [PMID: 36445780] (IHC, Mouse) IHC Mouse
MD Lauver, G Jin, KN Ayers, SN Carey, CS Specht, CS Abendroth, AE Lukacher T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus Elife, 2022-11-07;11(0):. 2022-11-07 [PMID: 36341713] (IHC, Mouse) IHC Mouse
Khalil R, Simitsidellis I, Kim NR et al. Androgen action on renal calcium and phosphate handling: Effects of bisphosphonate treatment and low calcium diet Molecular and Cellular Endocrinology 8/1/2020 [PMID: 32533994]
Xu, C, Wang, L Et al. Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms. J Am Soc Nephrol 5/1/2020 [PMID: 32253273]
T Fujiki, F Ando, K Murakami, K Isobe, T Mori, K Susa, N Nomura, E Sohara, T Rai, S Uchida Tolvaptan activates the Nrf2/HO-1 antioxidant pathway through PERK phosphorylation Sci Rep, 2019-06-25;9(1):9245. 2019-06-25 [PMID: 31239473] (Western Blot, Mouse) Western Blot Mouse
N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development Dev. Cell, 2017-05-22;41(4):349-365.e3. 2017-05-22 [PMID: 28535371] (IHC-Fr, Mouse) IHC-Fr Mouse
Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle PLoS ONE, 2016-07-15;11(7):e0158977. 2016-07-15 [PMID: 27420727] (IHC-P, Mouse) IHC-P Mouse
Matsumura , Takuhiro, Sugawara , Yo, Yutani , Masahiro, Amatsu , Sho, Yagita , Hideo, Kohda , Tomoko, Fukuoka , Shin-Ich, Nakamura , Yutaka, Fukuda , Shinji, Hase , Koji, Ohno , Hiroshi, Fujinaga , Yukako Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity. Nat Commun, 2015-02-17;6(0):6255. 2015-02-17 [PMID: 25687350] (IHC-Fr, Mouse) IHC-Fr Mouse

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Bioinformatics

Gene Symbol UMOD
Uniprot