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Uromodulin Antibodies

We offer Uromodulin Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Uromodulin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Uromodulin Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the Uromodulin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Uromodulin polyclonal antibodies and browse our Uromodulin monoclonal antibody catalog.

Alternate Names for Uromodulin Antibodies

anti-Uromodulin antibody, anti-UMOD antibody, anti-ADMCKD2 antibody, anti-FJHN antibody, anti-HNFJ antibody, anti-HNFJ1 antibody, anti-MCKD2 antibody, anti-Tamm-Horsfall glycoprotein antibody, anti-Tamm-Horsfall urinary glycoprotein antibody, anti-THGP antibody, anti-THP antibody, anti-uromodulin (uromucoid, Tamm-Horsfall glycoprotein) antibody, anti-uromucoid antibody

9 Results for "uromodulin" in Primary Antibodies

AF5175

Uromodulin Antibody [Unconjugated]

(8 Publications)

100 μg

AF5175

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western shows lysates of mouse kidney, loaded at 0.5 mg/ml. A specific band was detected for Uromodulin at approximately 182 kDa (as indicated) using 20 µg/mL of Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder and left and right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) and uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) and merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pax8-CreERT2 mediated beta -galactosidase expression.(A) Enzymatic X-gal staining of cryosections of kidneys (top panels) derived from 10 week old Pax8-CreERT2/Rosa26R mice either induced with tamoxifen (a, renal parenchyma; b, collecting ducts) or uninduced (c). Tamoxifen administration did not induce beta -galactosidase expression in any of the other major organs examined (representative tissues presented in bottom panels). (B) beta -galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules—AQP1, distal tubules–THP and collecting ducts–AQP2). Scale bars, 100μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26866916), licensed under a CC-BY license. Not internally tested by R&D Systems.

Slc22a6-CreERT2 mediated beta -galactosidase expression.(A) Enzymatic X-gal staining of cryosections of kidneys (top panels) derived from 10 week old Slc22a6-CreERT2/Rosa26R mice either induced with tamoxifen (a, renal cortex; b, medulla) or uninduced (c, renal cortex). Tamoxifen administration did not induce beta -galactosidase expression in any of the other major organs examined (representative tissues presented in bottom panels). (B) beta -galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26866916), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF5175

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5175-SP

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MAB5175

Uromodulin Antibody (774056)

(8 Publications)

100 μg

MAB5175

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5175-SP

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AF5144

Uromodulin Antibody

(4 Publications)

100 μg

AF5144

Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Uromodulin at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Uromodulin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF5144

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5144-SP

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MAB5144

Uromodulin Antibody (877914)

(3 Publications)

100 μg

MAB5144

Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Uromodulin Monoclonal Antibody (Catalog # MAB5144) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Uromodulin at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.

Uromodulin was detected in formalin fixed paraffin-embedded sections of human ovary tissue using Mouse Anti-Human Uromodulin Monoclonal Antibody (Catalog # MAB5144) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5144-SP

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H00007369-B01P

Uromodulin Antibody

(2 Publications)

0.05 mg

H00007369-B01P

Western Blot: Uromodulin Antibody [H00007369-B01P] - Analysis of UMOD expression in transfected 293T cell line by UMOD polyclonal antibody. Lane 1: UMOD transfected lysate(67.21 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Uromodulin Antibody [H00007369-B01P] - Analysis of purified antibody to UMOD on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: Uromodulin Antibody [H00007369-B01P] - Analysis of purified antibody to UMOD on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Polyclonal

Host: Mouse Polyclonal

Conjugate:

Unconjugated

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NBP2-33393

Uromodulin Antibody

0.1 ml

NBP2-33393

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining in human kidney and cerebral cortex tissues using NBP2-33393 antibody. Corresponding UMOD RNA-seq data are presented for the same tissues.

<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human cerebral cortex, kidney, placenta and testis using Anti-UMOD antibody NBP2-33393 (A) shows similar protein distribution across tissues to independent antibody NBP2-33730 (B).

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-33730

Uromodulin Antibody

0.1 ml

NBP2-33730

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining in human kidney and cerebral cortex tissues using NBP2-33730 antibody. Corresponding UMOD RNA-seq data are presented for the same tissues.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human cerebral cortex shows no positivity in neurons as expected.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human cerebral cortex, kidney, placenta and testis using Anti-UMOD antibody NBP2-33730 (A) shows similar protein distribution across tissues to independent antibody NBP2-33393 (B).

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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H00007369-M03

Uromodulin Antibody (3F10)

(3 Publications)

0.1 mg

H00007369-M03

Western Blot: Uromodulin Antibody (3F10) [H00007369-M03] - Analysis of UMOD expression in transfected 293T cell line by UMOD monoclonal antibody (M03), clone 3F10. Lane 1: UMOD transfected lysate (Predicted MW: 66.7 KDa). Lane 2: Non-transfected lysate.

Western Blot: Uromodulin Antibody (3F10) [H00007369-M03] - Analysis of UMOD transfected lysate using anti-UMOD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UMOD rabbit polyclonal antibody.

ELISA: Uromodulin Antibody (3F10) [H00007369-M03] - Detection limit for recombinant GST tagged UMOD is 0.3 ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-58867

Uromodulin Antibody

100 ul

NBP1-58867

Western Blot: Uromodulin Antibody [NBP1-58867] - THP-1 cell lysate, concentration 0.2-1 ug/ml.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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