![Western Blot: UBR2 Antibody [NBP1-45243] - CD9-positive EVs induce catabolic response in C2C12 myotubes. EVs isolated from LCM were subjected to immunoprecipitation (IP) against CD9 with pre-immune IgG as control. Resulted pellet and supernatant (Sup) were used to treat C2C12 myotubes and compared with PBS and input EVs for catabolic activity using western blotting. Data (n=3) were analyzed by analysis of variance (ANOVA) and * denotes a difference from PBS treatment (P<0.05). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-017-00726-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UBR2-Antibody-Western-Blot-NBP1-45243-img0002.jpg "Western Blot: UBR2 Antibody [NBP1-45243] - CD9-positive EVs induce catabolic response in C2C12 myotubes. EVs isolated from LCM were subjected to immunoprecipitation (IP) against CD9 with pre-immune IgG as control. Resulted pellet and supernatant (Sup) were used to treat C2C12 myotubes and compared with PBS and input EVs for catabolic activity using western blotting. Data (n=3) were analyzed by analysis of variance (ANOVA) and * denotes a difference from PBS treatment (P<0.05). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-017-00726-x), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence SCPKSGGDKSRAPT corresponding to internal region according to NP_056070.1. |
| Epitope | SCPKSGGDKSRAPT |
| Predicted Species | Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | UBR2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Preliminary experiments gave bands at approx. 150 kDa band in rat testis lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 199 kDa band according to rat NP_001171542.1. The detected band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 09/06/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | UBR2 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/UBR2-Antibody-Immunohistochemistry-Paraffin-NBP1-45243-img0001.jpg "Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![UBR2 Antibody [NBP1-45243] - Hsp70 and 90-deficient LLC tumor does not induce muscle catabolism in mice. TA of mice derived from b was analyzed by western blotting for markers of catabolic pathways. Data (n=6) were analyzed by ANOVA. * denotes a difference (P<0.05). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-017-00726-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UBR2-Antibody-NBP1-45243-img0003.jpg "UBR2 Antibody [NBP1-45243] - Hsp70 and 90-deficient LLC tumor does not induce muscle catabolism in mice. TA of mice derived from b was analyzed by western blotting for markers of catabolic pathways. Data (n=6) were analyzed by ANOVA. * denotes a difference (P<0.05). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-017-00726-x), licensed under a CC-BY license.")
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