Novus Biologicals products are now on bio-techne.com

Histone H2a Antibody

Images

 
Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium ...read more
Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.

Product Details

Summary
Reactivity Hu, Mu, ShSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Histone H2a Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acid residues 1-15 (MSGRGKQGGKARAKA) and 81-96 (PRHLQLAIRNDEELNK) of human Histone H2A.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AC20
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:2000
  • Flow Cytometry reported in scientific literature (PMID 32849530)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2 ug/ml
Application Notes
A 14 kDa band is observed.
Reviewed Applications
Read 1 Review rated 4
using
NB100-56346 in the following applications:

Publications
Read Publications using
NB100-56346 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Use in Sheep reported in scientific literature (PMID:32849530).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Histone H2a Antibody

  • H2A histone family, member Q
  • H2A/q
  • H2A1B
  • H2AFQH2A
  • H2A-GL101
  • HIST1H2AB
  • histone 2, H2ac
  • histone cluster 2, H2ac
  • histone H2A type 2-C
  • Histone H2A
  • Histone H2A/q
  • Histone H2A-GL101
  • histone IIa
  • MGC74460

Background

Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-52486
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-07996
Species: Hu
Applications: WB
NLS3775
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NBP1-85351
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-45184
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00008294-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBL1-11570
Species: Hu
Applications: WB
H00008365-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA
NBL1-11567
Species: Hu
Applications: WB
AF5215
Species: Hu, Mu
Applications: ICC, WB
NB500-171
Species: Hu, I, Mu, Rt, Xp, Ye
Applications: ChIP, IB, ICC/IF, IHC, IHC-P, Single-Cell Western, WB
NBP2-67753
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP3-13389
Species: Hu
Applications: IHC, IHC-P, WB
AF2288
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Supplier Logo

Publications for Histone H2a Antibody (NB100-56346)(4)

Review for Histone H2a Antibody (NB100-56346) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56346:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Histone H2a NB100-56346
Enlarge
4
reviewed by:
Verified Customer
WB Human 07/31/2014
View

Summary

ApplicationWestern Blot
Sample TestedMCF-7 and HCT116 cells whole cell extract
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Histone H2a Antibody (NB100-56346) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Histone H2a Products

Research Areas for Histone H2a Antibody (NB100-56346)

Find related products by research area.

Blogs on Histone H2a

There are no specific blogs for Histone H2a, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
07/31/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol H2AC20
Entrez
Uniprot