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UAP56 Antibody (OTI2C5) - Azide and BSA Free

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Western Blot: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UAP56 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UAP56.
Western Blot: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UAP56 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UAP56 mouse monoclonal antibody.
Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UAP56 mouse monoclonal antibody.
Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727] - Staining of paraffin-embedded Human prostate tissue using anti-UAP56 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74727AF350
Alexa Fluor 405
NBP2-74727AF405
Alexa Fluor 488
NBP2-74727AF488
Alexa Fluor 532
NBP2-74727AF532
Alexa Fluor 594
NBP2-74727AF594
Alexa Fluor 647
NBP2-74727AF647
Alexa Fluor 700
NBP2-74727AF700
Alexa Fluor 750
NBP2-74727AF750
Biotin
NBP2-74727B
CoraFluor 1
NBP2-74727CL1
DyLight 350
NBP2-74727UV
DyLight 405
NBP2-74727V
DyLight 488
NBP2-74727G
DyLight 550
NBP2-74727R
DyLight 594
NBP2-74727DL594
DyLight 650
NBP2-74727C
DyLight 680
NBP2-74727FR
DyLight 755
NBP2-74727IR
FITC
NBP2-74727F
HRP
NBP2-74727H
Janelia Fluor 525
NBP2-74727JF525
Janelia Fluor 549
NBP2-74727JF549
Janelia Fluor 585
NBP2-74727JF585
Janelia Fluor 635
NBP2-74727JF635
Janelia Fluor 646
NBP2-74727JF646
Janelia Fluor 669
NBP2-74727JF669
Unconjugated
NBP2-02553
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02553
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UAP56 Antibody (OTI2C5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human BAT1(NP_542165) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DDX39B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Theoretical MW
48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for UAP56 Antibody (OTI2C5) - Azide and BSA Free

  • 56 kDa U2AF65-associated protein
  • ATP-dependent RNA helicase p47
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
  • DEAD box protein UAP56
  • EC 3.6.4.13
  • HLA-B associated transcript 1
  • HLA-B-associated transcript 1 protein
  • spliceosome RNA helicase BAT1
  • UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Background

UAP56 encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DDX39B