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u-Plasminogen Activator/Urokinase Antibody (204212) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, ELISA(Cap)
Clone
204212
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1310-SP
Carrier Free
MAB1310-500
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u-Plasminogen Activator/Urokinase Antibody (204212) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human u-Plasminogen Activator (uPA)/Urokinase Catalytic Domain
Ser21-Leu431
Accession # P00749
Specificity
Detects human u-Plasminogen Activator (uPA)/Urokinase Catalytic Domain in direct ELISAs and Western blots. In Western blots under reducing conditions, it reacts with the catalytic domain (B chain) only. In Western blots under non-reducing conditions, it reacts with the B chain and disulfide bond-linked B and A chains. In direct ELISAs, no cross-reactivity with recombinant human (rh) Factor X, rhHGFA, rhKLK‑3, -4, -5, -6, -8, -10, -11, rhThrombin, or recombinant mouse WIF-1 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PLAU
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human u-Plasminogen Activator (uPA)/Urokinase Catalytic Domain Antibody (Catalog # MAB1310)
ELISA Detection: Human u-Plasminogen Activator (uPA)/Urokinase Biotinylated Antibody (Catalog # BAF1310)
Standard: Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE)
Publications
Read Publications using
MAB1310 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for u-Plasminogen Activator/Urokinase Antibody (204212) [Unconjugated]

  • ATF
  • EC 3.4.21
  • EC 3.4.21.73
  • plasminogen activator, urokinase
  • PLAU
  • uPA
  • u-PA
  • uPlasminogen Activator
  • u-Plasminogen Activator
  • urinary
  • Urokinase
  • urokinase-type plasminogen activator

Background

uPA is a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin (1). uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium (2). For example, the combination (both low vs. either or both high) of uPA and its inhibitor, plasminogen activator inhibitor-1 (PAI-1), outperforms the single factors as well as other traditional prognostic factors with regard to risk group assessment for breast cancer, particularly in node-negative breast cancer (3). The human uPA is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues) (4‑6). The single chain molecule is processed into a disulfide-linked two-chain molecule. The B chain starting at Ile179 corresponds to the catalytic domain. Two forms of the A chain exist, one starting at Ser21 (the long form) and the other at Lys156 (the short form). The resulting two-chain forms have different molecular weights (MW). The B chain is common for both forms whereas the long and short A chains are unique to the high and low MW forms, respectively. The long A chain contains an EGF-like domain, which is responsible for binding of the uPA receptor (uPAR).

  1. Ellis, V. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds., Academic Press, San Diego, pp.1677.
  2. Duffy, M.J. (2002) Biochem. Soc. Trans. 30:207.
  3. Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196.
  4. Riccio, A. et al. (1985) Nucleic Acids Res. 13:2785.
  5. Nagai, M. et al. (1985) Gene 36:183.
  6. Jacobs, P. et al. (1985) DNA 4:139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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u-Plasminogen Activator/Urokinase Antibody (204212) [Unconjugated]
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Publications for u-Plasminogen Activator/Urokinase Antibody (MAB1310)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(3)
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Human
(3)
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Showing Publications 1 - 5 of 5.
Publications using MAB1310 Applications Species
Wenjun Peng, Yuanyuan Wu, Ge Zhang, Wensi Zhu, Meijia Chang, Ainiwaer Rouzi et al. GLIPR1 Protects Against Cigarette Smoke-Induced Airway Inflammation via PLAU/EGFR Signaling International Journal of Chronic Obstructive Pulmonary Disease 10/8/2021 [PMID: 34675506]
Huajiao Guo, Fuhao Wang, Yuwen Diao, Zhe Zhang, Qiuyan Chen, Chao‐Nan Qian et al. Knockdown of Notch1 inhibits nasopharyngeal carcinoma cell growth and metastasis via downregulation of CCL2, CXCL16, and uPA Molecular Carcinogenesis 2019-10-01 [PMID: 31270884]
Devine D, Rostas J, Metge B, Das S, Mulekar M, Tucker J, Grizzle W, Buchsbaum D, Shevde L, Samant R Loss of N-Myc interactor promotes epithelial-mesenchymal transition by activation of TGF-beta/SMAD signaling. Oncogene, 2013-06-17;33(20):2620-8. 2013-06-17 [PMID: 23770854] (Western Blot, Human) Western Blot Human
Biadasiewicz K, Sonderegger S, Haslinger P, Haider S, Saleh L, Fiala C, Pollheimer J, Knofler M Transcription Factor AP-2alpha Promotes EGF-Dependent Invasion of Human Trophoblast. Endocrinology, 2011-02-08;152(4):1458-69. 2011-02-08 [PMID: 21303946] (Western Blot, Human) Western Blot Human
Schnickmann S, Camacho-Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, Szabowski A, Breuhahn K AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor. J. Invest. Dermatol., 2008-11-20;129(5):1140-8. 2008-11-20 [PMID: 19020551] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol PLAU
Uniprot