Novus Biologicals products are now on bio-techne.com

TSHR Antibody (SPM222) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: TSH R Antibody (SPM222) [NBP2-54317] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with TSHRB Monoclonal Antibody (SPM222).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM222
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

TSHR Antibody (SPM222) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53224).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human TSHR protein (Uniprot: P16473)
Localization
Cell Surface & Cytoplasmic
Marker
Thyroid Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TSHR
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for TSHR Antibody (SPM222) - Azide and BSA Free

  • CHNG1
  • LGR3
  • LGR3hTSHR-I
  • MGC75129
  • seven transmembrane helix receptor
  • thyroid stimulating hormone receptor
  • Thyroid-stimulating hormone receptor
  • thyrotropin receptor
  • thyrotropin receptor-I, hTSHR-I
  • TSH R
  • TSHR
  • TSH-R

Background

Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
NBP1-80670
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-44501
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IF, IHC, IHC-P
288-TPN/CF
Species: Hu
Applications: BA
7268-CT
Species: Hu
Applications: BA
NLS1436
Species: Bv, Ca, Hu
Applications: IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-38770
Species: Hu, Mu
Applications: IHC-P, WB
NB110-41404
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NBP3-27122
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-36489
Species: Hu
Applications: IHC, IHC-P, WB
6507-IL/CF
Species: Hu
Applications: BA
NBP1-32440
Species: Ca, Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, Simple Western, Single-Cell Western, WB
MAB8458
Species: Hu
Applications: CyTOF-ready, Flow, ICC
NBP1-89781
Species: Hu
Applications: IHC, IHC-P, WB

Publications for TSHR Antibody (NBP2-54317) (0)

There are no publications for TSHR Antibody (NBP2-54317).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TSHR Antibody (NBP2-54317) (0)

There are no reviews for TSHR Antibody (NBP2-54317). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for TSHR Antibody (NBP2-54317) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TSHR Products

Research Areas for TSHR Antibody (NBP2-54317)

Find related products by research area.

Blogs on TSHR

There are no specific blogs for TSHR, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TSHR Antibody (SPM222) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol TSHR