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Tryptophan Hydroxylase 1/TPH-1 Antibody [Unconjugated]

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Western blot shows lysates of AGS human gastric adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tryptophan Hydroxylase 1/TPH-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Tryptophan Hydroxylase 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of AGS human gastric adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tryptophan Hydroxylase 1/TPH-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Tryptophan Hydroxylase 1/TPH‑1 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human Tryptophan Hydroxylase 1/TPH‑1 Antigen ...read more
Tryptophan Hydroxylase 1/TPH-1 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Human Tryptophan Hydroxylase 1/TPH-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5276) at 1.7 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5276-SP
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Tryptophan Hydroxylase 1/TPH-1 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tryptophan Hydroxylase 1/TPH‑1
Met102-Pro403
Accession # P17752
Specificity
Detects human Tryptophan Hydroxylase 1/TPH‑1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TPH1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5276 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tryptophan Hydroxylase 1/TPH-1 Antibody [Unconjugated]

  • EC 1.14.16
  • EC 1.14.16.4
  • indoleacetic acid-5-hydroxylase
  • L-tryptophan hydroxylase
  • MGC119994
  • TPH
  • TPH-1
  • TPRH
  • TRPH
  • tryptophan 5-hydroxylase 1
  • Tryptophan 5-monooxygenase 1
  • tryptophan hydroxylase (tryptophan 5-monooxygenase)
  • Tryptophan Hydroxylase 1

Background

Tryptophan Hydroxylase 1 (TPH-1) is a 50‑60 kDa intracellular enzyme that belongs to the biopterin-dependent, aromatic amino acid hydroxylase family. It is expressed in gut enterochromaffin cells and pineal gland. TPH-1 catalyzes the hydroxylation of L-Trp, which is the rate-limiting step in serotonin biosynthesis. Human TPH-1 is 444 amino acids (aa) in length. It contains an N-terminal regulatory domain (aa 1‑98), a catalytic region (aa 99‑424), and a C‑terminal Leu-zipper tetramerization domain (aa 425‑444). TPH-1 is phosphorylated on Ser58, and subsequently binds to 14-3-3 proteins, resulting in activation. It functions as a noncovalent Fe-binding 230 kDa homotetramer. There are three potential splice variants. One shows a 29 aa substitution for aa 438‑444, a second shows a 50 aa substitution for aa 157‑444, and a third shows a 68 aa substitution for aa 1‑21. Over aa 102‑403, Human TPH-1 shares 95% aa identical with mouse TPH-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tryptophan Hydroxylase 1/TPH-1 Antibody (AF5276)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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Bioinformatics

Gene Symbol TPH1
Uniprot