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Trypsin 2/PRSS2 Antibody (410322) [Unconjugated]

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Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Trypsin 2/PRSS2 at approximately 24 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586) followed by HRP-conjugated Anti-Mouse IgG ...read more
Western blot shows recombinant human Trypsin 1, recombinant human Trypsin 2 and recombinant human Trypsin 3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin-2/PRSS2 Monoclonal Antibody (Catalog # ...read more
Human Trypsin 2/PRSS2 was immunoprecipitated from 500 μg of human pancreas lysates with 12.5 ug Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586). The Trypsin 2/PRSS2-antibody complexes ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
410322
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB3586-SP
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Trypsin 2/PRSS2 Antibody (410322) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trypsin 2/PRSS2
Ala16-Ser247
Accession # P07478
Specificity
Detects human Trypsin 2/PRSS2 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Trypsin 1 or rhTrypsin 3. Recognizes both the pro and mature forms of rhTrypsin 2.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRSS2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 12.5 ug/500 ug cell lysate
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB3586 in the following applications:
Publications
Read Publication using
MAB3586 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trypsin 2/PRSS2 Antibody (410322) [Unconjugated]

  • Anionic trypsinogen
  • EC 3.4.21
  • EC 3.4.21.4
  • MGC111183
  • MGC120174
  • MGC120175
  • protease serine 2 preproprotein
  • protease, serine, 2 (trypsin 2)
  • protease, serine, 2, preproprotein
  • PRSS2
  • Serine protease 2
  • Tesp4
  • TRY2TRY8
  • TRY8
  • TRYP2
  • Trypsin 2
  • Trypsin II
  • trypsin-2
  • trypsinogen 2
  • Trypsinogen 20

Background

Human Trypsin 2, encoded by the PRSS2 gene, is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).

  1. Emi, M. et al. (1986) Gene. 41:305.
  2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego.
  3. Rinderknecht, H. et al. (1979) Gut. 20:886.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Trypsin 2/PRSS2
Antibody (410322) [Un ...
MAB3586
Trypsin 2/PRSS2 Antibody (410322) [Unconjugated]
Species: Hu
Applications: WB, IP

Publications for Trypsin 2/PRSS2 Antibody (MAB3586)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Review for Trypsin 2/PRSS2 Antibody (MAB3586) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB3586:
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ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 ICC Human 02/20/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 20407433
SpeciesHuman

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Recent Reviews

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 Verified Customer
02/20/2015
Application: ICC
Species: Human
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Bioinformatics

Gene Symbol PRSS2
Uniprot