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Troponin I Type 3 (cardiac) Antibody (4C2cc) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Fe, Gt, RbSpecies Glossary
Applications WB, ELISA, IHC, IP, AP
Clone
4C2cc
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Troponin I Type 3 (cardiac) Antibody (4C2cc) [DyLight 488] Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI.
Epitope
a.a.r. 23-29
Specificity
No cross-reaction with skeletal muscle TnI.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TNNI3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Immunoassay
  • Immunohistochemistry
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Troponin I Type 3 (cardiac) Antibody (4C2cc) [DyLight 488]

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • CTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1
  • TNNC1troponin I, cardiac
  • TNNI3,
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle
  • Troponin I/TNNI3

Background

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI3