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Troponin I type 2 (fast skeletal) Antibody

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Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26201] - Staining of Human (A), Mouse(B) and Rat (C) Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Troponin I type 2 (fast skeletal) Antibody Summary

Immunogen
Peptide with sequence C-EVRVQKTSKELED corresponding to internal region according to NP_003273.1, NP_001139313.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNNI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human, mouse and rat skeletal muscle lysates (calculated MW of 21.3 kDa band according to NP_003273.1).
Publications
Read Publication using NBP2-26201.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Troponin I type 2 (fast skeletal) Antibody

  • skeletal, fast
  • troponin I type 2 (skeletal, fast)
  • troponin I, fast skeletal muscle
  • Troponin I, fast-twitch isoform
  • troponin I, fast-twitch skeletal muscle isoform

Background

The troponin I type 2 gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Troponin I type 2 (fast skeletal) Antibody (NBP2-26201)(1)

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Troponin I type 2 (fast skeletal) Antibody (NBP2-26201)

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Blogs on Troponin I type 2 (fast skeletal).

Troponin I Type 2 - I stay with fast-twitch skeletal muscles only
The protein Troponin I is a component of the heteromeric protein complex responsible for regulating both skeletal and cardiac muscle contraction. Troponin complex is made up of three parts: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI2
Entrez