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Triosephosphate isomerase Antibody (1D10-2E2)

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Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - TPI1 monoclonal antibody (M01), clone 1D10-2E2 Analysis of TPI1 expression in HepG2.
Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - Antibody Reactive Against Recombinant TPI1 (AAH07086, 1 a.a. ~ 250 a.a) ) full length protein with GST tag (53.5 KDa) .
Sandwich ELISA: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - Detection limit for recombinant GST tagged FLJ23834 is approximately 0.1ng/ml as a capture antibody.
Sandwich ELISA: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - Detection limit for recombinant GST tagged TPI1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1D10-2E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Triosephosphate isomerase Antibody (1D10-2E2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
TPI1 (AAH07086.1, 1 a.a. ~ 249 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDTINAKQ
Localization
Cytoplasmic
Specificity
TPI1 - triosephosphate isomerase 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TPI1
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Publications
Read Publications using H00007167-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Triosephosphate isomerase Antibody (1D10-2E2)

  • EC 5.3.1.1
  • MGC88108
  • TIM
  • TPI
  • triosephosphate isomerase 1
  • triosephosphate isomerase
  • Triose-phosphate isomerase

Background

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TPI1
Entrez
OMIM
Uniprot