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TRIB2 Antibody (OTI8D11) - Azide and BSA Free

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Western Blot: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TRIB2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Staining of COS7 cells using anti-TRIB2 mouse monoclonal antibody .
Immunohistochemistry: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Staining of paraffin-embedded Human endometrium tissue using anti-TRIB2 mouse monoclonal antibody.
Flow Cytometry: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TRIB2 ...read more
Immunohistochemistry: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRIB2 mouse monoclonal antibody.
Immunohistochemistry: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIB2 mouse monoclonal antibody.
Immunohistochemistry: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRIB2 mouse monoclonal antibody.
Immunohistochemistry: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRIB2 mouse monoclonal antibody.
Immunohistochemistry: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TRIB2 mouse monoclonal antibody.
Immunohistochemistry: TRIB2 Antibody (OTI8D11) - Azide and BSA Free [NBP2-74597] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRIB2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI8D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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TRIB2 Antibody (OTI8D11) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human TRIB2 (NP_067675) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TRIB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
Theoretical MW
38.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TRIB2 Antibody (OTI8D11) - Azide and BSA Free

  • C5FW
  • FLJ57420
  • GS3955
  • TRB-2
  • TRB2tribbles homolog 2
  • tribbles homolog 2 (Drosophila)

Background

The TRIB2 gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIB2