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TRIB1 Antibody

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Immunohistochemistry-Paraffin: TRIB1 Antibody [NBP1-20988] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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TRIB1 Antibody Summary

Immunogen
Peptide with sequence C-TSDQIVPEYQEDSD corresponding to C-Terminus according to NP_079471.1 .
Epitope
C terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx 35kDa band in Human Lymph Node and Peripheral Blood Mononucleocyte lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.0kDa according to NP_079471.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Spleen.
Publications
Read Publication using NBP1-20988.

Reactivity Notes

Expected from sequence similarity: Mouse and Bovine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIB1 Antibody

  • C8FWG-protein-coupled receptor induced protein
  • GIG-2
  • GIG2G-protein-coupled receptor-induced protein 2
  • G-protein-coupled receptor-induced gene 2 protein
  • SKIP1
  • TRB-1
  • TRB1phosphoprotein regulated by mitogenic pathways
  • tribbles homolog 1 (Drosophila)
  • tribbles homolog 1
  • tribbles-like protein 1

Background

Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIB1 Antibody (NBP1-20988)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIB1
Entrez
Uniprot