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Transglutaminase 2/TGM2 Antibody

Images

 
Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-20698] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: Transglutaminase 2/TGM2 Antibody [NBP2-20698] - Immunofluorescence analysis of methanol-fixed A549, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-20698] - Mouse duodenum. Transglutaminase 2 antibody [N1N3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-20698] - Mahlarvu xenograft, using Transglutaminase 2 antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Transglutaminase 2/TGM2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Transglutaminase 2/TGM2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGM2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-20698 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%), Guinea pig (85%), Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Transglutaminase 2/TGM2 Antibody

  • C polypeptide
  • EC 2.3.2.13
  • G-ALPHA-h
  • protein-glutamine gamma-glutamyltransferase 2
  • protein-glutamine-gamma-glutamyltransferase
  • TG(C)
  • TG2
  • TGase C
  • TGase H
  • TGase-2
  • TGase-H
  • TGC
  • TGCGNAH
  • TGM2
  • Tissue transglutaminase
  • transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
  • Transglutaminase 2
  • Transglutaminase C
  • Transglutaminase H
  • transglutaminase-2
  • tTG

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Transglutaminase 2/TGM2 Antibody (NBP2-20698)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TGM2
Uniprot