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TPRKB Antibody (OTI3H3)

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Western Blot: TPRKB Antibody (3H3) [NBP2-03885] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-TPRKB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: TPRKB Antibody (3H3) [NBP2-03885] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TPRKB.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Human tonsil using anti-TPRKB mouse monoclonal antibody.
Flow Cytometry: TPRKB Antibody (3H3) [NBP2-03885] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TPRKB antibody, and then analyzed ...read more
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Human colon tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Human Kidney tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Human liver tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Human lymphoma tissue using anti-TPRKB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TPRKB Antibody (3H3) [NBP2-03885] - Staining of paraffin-embedded Human pancreas tissue using anti-TPRKB mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TPRKB Antibody (OTI3H3) Summary

Immunogen
Full length human recombinant protein of human TPRKB (NP_057142) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TPRKB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-1000
Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TPRKB Antibody (OTI3H3)

  • CGI-121
  • PRPK (p53-related protein kinase)-binding protein
  • PRPK-binding protein
  • TP53RK binding protein
  • TP53RK-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TPRKB
Entrez