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TIF1 alpha Antibody (PCRP-TRIM24-1B12) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, MA
Clone
PCRP-TRIM24-1B12
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08786AF350
Alexa Fluor 405
NBP3-08786AF405
Alexa Fluor 488
NBP3-08786AF488
Alexa Fluor 532
NBP3-08786AF532
Alexa Fluor 594
NBP3-08786AF594
Alexa Fluor 647
NBP3-08786AF647
Alexa Fluor 700
NBP3-08786AF700
Alexa Fluor 750
NBP3-08786AF750
Allophycocyanin
NBP3-08786APC
Biotin
NBP3-08786B
DyLight 350
NBP3-08786UV
DyLight 405
NBP3-08786V
DyLight 488
NBP3-08786G
DyLight 550
NBP3-08786R
DyLight 594
NBP3-08786DL594
DyLight 650
NBP3-08786C
DyLight 680
NBP3-08786FR
DyLight 755
NBP3-08786IR
FITC
NBP3-08786F
HRP
NBP3-08786H
Janelia Fluor 525
NBP3-08786JF525
Janelia Fluor 549
NBP3-08786JF549
Janelia Fluor 585
NBP3-08786JF585
Janelia Fluor 635
NBP3-08786JF635
Janelia Fluor 646
NBP3-08786JF646
Janelia Fluor 669
NBP3-08786JF669
PE
NBP3-08786PE
PerCP
NBP3-08786PCP
Unconjugated
NBP3-07464-100ug
mFluor Violet 450 SE
NBP3-08786MFV450
mFluor Violet 500 SE
NBP3-08786MFV500
mFluor Violet 610 SE
NBP3-08786MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07464-100ug
Azide and BSA Free
NBP3-08786
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TIF1 alpha Antibody (PCRP-TRIM24-1B12) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant full-length human TIF1 alpha protein (Uniprot: O15164)
Localization
Nucleus, Cytoplasm. Colocalizes with sites of active transcription.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRIM24
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Protein Array
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TIF1 alpha Antibody (PCRP-TRIM24-1B12) [CoraFluor™ 1]

  • E3 ubiquitin-protein ligase TRIM24
  • EC 6.3.2
  • EC 6.3.2.-
  • hTIF1
  • PTC6
  • RING finger protein 82
  • RNF82
  • RNF82Tif1a
  • TIF1 alpha
  • TIF1
  • TIF1A
  • TIF1-alpha
  • TIF1ATIF1ALPHA
  • TIF1TF1A
  • transcription intermediary factor 1-alpha
  • transcriptional intermediary factor 1
  • TRIM24
  • tripartite motif containing 24
  • tripartite motif-containing 24
  • Tripartite motif-containing protein 24

Background

TIF1 alpha is encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TIF1 alpha
Antibody (PCRP-TRIM24 ...
NBP3-08786CL1
TIF1 alpha Antibody (PCRP-TRIM24-1B12) [CoraFluor™ 1]
Species: Hu
Applications: Flow, ICC/IF, MA

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM24