Thymidylate Synthase Antibody (TS106) - Azide and BSA Free Summary
Description |
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-34445).
Antibody with azide - store at 2 to 8C. |
Immunogen |
Recombinant full-length human Thymidylate Synthase protein. (Uniprot: P04818 ) |
Localization |
Cytoplasm, Nucleus, Mitochondria |
Marker |
5-FU Resistance Marker |
Isotype |
IgG1 Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
TYMS |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry
- Immunocytochemistry/ Immunofluorescence 0.5-1ug/ml
- Immunohistochemistry
- Immunohistochemistry-Paraffin 0.5-1ug/ml
|
Application Notes |
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Theoretical MW |
36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer |
10 mM PBS |
Preservative |
No Preservative |
Concentration |
1.0 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for Thymidylate Synthase Antibody (TS106) - Azide and BSA Free
Background
Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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