Thrombospondin-3 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

THBS3 is a gene that codes for an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and has two isoforms, with lengths of 956 and 836 amino acids and weights of approximately 104 and 91 kDa respectively. Current research is being done on diseases and disorders related to this gene including osteosarcoma, lung cancer, breast cancer, retinitis, malaria, neuronitis, and echinococcosis. THBS3 has also been shown to have interactions with THBS2, THBS1, THBS4, FURIN, and PDGFB in pathways such as the focal adhesion, inflammatory response, signal transduction, signaling by PDGF, phagosome, ECM-receptor interaction, and malaria pathways.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-07529

• Thrombospondin-3 Antibodies
• Thrombospondin-3 Lysate
• Thrombospondin-3 Protein
• Thrombospondin-3 Proteins and Enzyme