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TfR (Transferrin R) Antibody (TFRC/1817) - Azide and BSA Free

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Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (TFRC/1817) - Azide and BSA Free [NBP2-54467] - Formalin-fixed, paraffin-embedded Human Placenta stained

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, CyTOF-ready
Clone
TFRC/1817
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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TfR (Transferrin R) Antibody (TFRC/1817) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53274).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant extracellular fragment (around aa 94-212) of human TfR (Transferrin R) protein (exact sequence is proprietary) (Uniprot: P02786)
Localization
Cell surface
Specificity
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TFRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 2 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Western Blot 0.5 - 1.0 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for TfR (Transferrin R) Antibody (TFRC/1817) - Azide and BSA Free

  • CD71 antigen
  • CD71
  • CD71TRFR
  • p90
  • T9
  • TfR (Transferrin R)
  • TfR (TransferrinR)
  • TfR
  • TFR1
  • TFRC
  • TR
  • transferrin receptor (p90, CD71)
  • transferrin receptor protein 1
  • TRFR

Background

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TFRC