![Western Blot: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Western-Blot-NBP2-80976-img0001.jpg "Western Blot: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OX26 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | PHA activated rat lymphocytes. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TFRC |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for TfR (Transferrin R) Antibody (NBP2-80976)Find related products by research area.
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Transferrin and the blood brain barrier Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i... Read full blog post. |
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| Gene Symbol | TFRC |
![Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti TfR/Transferrin R (OX26) NB200-585 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (red) at a 1:200 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80976-img0002.jpg "Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti TfR/Transferrin R (OX26) NB200-585 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (red) at a 1:200 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis of FFPE mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenchi](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80976-img0003.jpg "Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis of FFPE mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenchi")
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen lymphocytes with mouse anti rat CD71. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80976-img0007.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen lymphocytes with mouse anti rat CD71. Image from the standard format of this antibody.")
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - An intracellular stain was performed on PC12 cells with TfR (Transferrin R) [OX26] Antibody NB200-585AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80976-img0004.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - An intracellular stain was performed on PC12 cells with TfR (Transferrin R) [OX26] Antibody NB200-585AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.")
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis using the Biotin conjugate of NB200-585. Staining of mouse spleen.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80976-img0005.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis using the Biotin conjugate of NB200-585. Staining of mouse spleen.")
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen cells with mouse anti rat CD71: RPE. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80976-img0006.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen cells with mouse anti rat CD71: RPE. Image from the standard format of this antibody.")
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