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TfR (Transferrin R) Antibody (DF1513) [CoraFluor™ 1]

 
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, CyTOF-ready
Clone
DF1513
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34602AF350
Alexa Fluor 405
NBP2-34602AF405
Alexa Fluor 488
NBP2-34602AF488
Alexa Fluor 532
NBP2-34602AF532
Alexa Fluor 594
NBP2-34602AF594
Alexa Fluor 647
NBP2-34602AF647
Alexa Fluor 700
NBP2-34602AF700
Alexa Fluor 750
NBP2-34602AF750
Allophycocyanin
NBP2-34602APC
Allophycocyanin/Cy7
NBP2-34602APCCY7
Biotin
NBP2-34602B
DyLight 350
NBP2-34602UV
DyLight 405
NBP2-34602V
DyLight 488
NBP2-34602G
DyLight 550
NBP2-34602R
DyLight 594
NBP2-34602DL594
DyLight 650
NBP2-34602C
DyLight 680
NBP2-34602FR
DyLight 755
NBP2-34602IR
FITC
NBP2-34602F
HRP
NBP2-34602H
Janelia Fluor 525
NBP2-34602JF525
Janelia Fluor 549
NBP2-34602JF549
Janelia Fluor 646
NBP2-34602JF646
Janelia Fluor 669
NBP2-34602JF669
PE
NBP2-34602PE
PE/Atto594
NBP2-34602PEATT594
PE/Cy5.5
NBP2-34602PECY55
PE/Cy7
NBP2-34602PECY7
PerCP
NBP2-34602PCP
Unconjugated
NBP2-32945-0.1mg
mFluor Violet 450 SE
NBP2-34602MFV450
mFluor Violet 500 SE
NBP2-34602MFV500
mFluor Violet 610 SE
NBP2-34602MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-32945-0.1mg
Azide and BSA Free
NBP2-34602-0.1mg
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TfR (Transferrin R) Antibody (DF1513) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Clone DF1513 was used by HLDA to establish CD designation.
Immunogen
KG1 acute myeloid leukemia cell line
Localization
Cell surface
Specificity
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TFRC
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TfR (Transferrin R) Antibody (DF1513) [CoraFluor™ 1]

  • CD71 antigen
  • CD71
  • CD71TRFR
  • p90
  • T9
  • TfR (Transferrin R)
  • TfR (TransferrinR)
  • TfR
  • TFR1
  • TFRC
  • TR
  • transferrin receptor (p90, CD71)
  • transferrin receptor protein 1
  • TRFR

Background

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TFRC