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TBCC Antibody (OTI4C4) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74467AF350
Alexa Fluor 405
NBP2-74467AF405
Alexa Fluor 488
NBP2-74467AF488
Alexa Fluor 532
NBP2-74467AF532
Alexa Fluor 594
NBP2-74467AF594
Alexa Fluor 647
NBP2-74467AF647
Alexa Fluor 700
NBP2-74467AF700
Alexa Fluor 750
NBP2-74467AF750
Biotin
NBP2-74467B
CoraFluor 1
NBP2-74467CL1
DyLight 350
NBP2-74467UV
DyLight 405
NBP2-74467V
DyLight 488
NBP2-74467G
DyLight 550
NBP2-74467R
DyLight 594
NBP2-74467DL594
DyLight 650
NBP2-74467C
DyLight 680
NBP2-74467FR
DyLight 755
NBP2-74467IR
FITC
NBP2-74467F
HRP
NBP2-74467H
Janelia Fluor 525
NBP2-74467JF525
Janelia Fluor 549
NBP2-74467JF549
Janelia Fluor 635
NBP2-74467JF635
Janelia Fluor 646
NBP2-74467JF646
Janelia Fluor 669
NBP2-74467JF669
Unconjugated
NBP2-02037
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02037
Azide and BSA Free
NBP2-74467
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TBCC Antibody (OTI4C4) [Janelia Fluor® 585] Summary

Immunogen
Full length human recombinant protein of human TBCC(NP_003183) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBCC
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TBCC Antibody (OTI4C4) [Janelia Fluor® 585]

  • CFCtubulin-specific chaperone c
  • tubulin folding cofactor C
  • Tubulin-folding cofactor C

Background

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
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Bioinformatics

Gene Symbol TBCC