![Western Blot: ARL2 Antibody [NB100-1078] - (0.5ug/ml) staining of A431 (A) and (1ug/ml) HeLa nuclear (B) cell lysate (30ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1078_goat-polyclonal-arl2-antibody-187202311342918.jpg "Western Blot: ARL2 Antibody [NB100-1078] - (0.5ug/ml) staining of A431 (A) and (1ug/ml) HeLa nuclear (B) cell lysate (30ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-LDDISSRIFTAD corresponding to C-Terminus according to NP_001658.2. |
| Predicted Species | Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ARL2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 18kDa band observed in lysates of cell line A431 and approx. 20kDa in nuclear lysates of cell line HeLa (calculated MW of 20.9kDa according to NP_001658.2). Primary incubation 1 hour at room temperature. |
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| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: ARL2 Antibody [NB100-1078] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-1078_goat-polyclonal-arl2-antibody-18720231134298.jpg "Immunocytochemistry/Immunofluorescence: ARL2 Antibody [NB100-1078] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Flow Cytometry: ARL2 Antibody [NB100-1078] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-1078_goat-polyclonal-arl2-antibody-187202311342930.jpg "Flow Cytometry: ARL2 Antibody [NB100-1078] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
