| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Concentration | 0.5 mg/ml |
| Description | A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-400 of Human TAZ/WWTR1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | WWTR1 |
| Purity | >85%, by SDS-PAGE |
| Dilutions |
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| Application Notes | Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
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| Theoretical MW | 46.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Purity | >85%, by SDS-PAGE |
| Publication using NBP2-22949 | Applications | Species |
|---|---|---|
| Wang D, Li Z, Li X et al. DUB1 suppresses Hippo signaling by modulating TAZ protein expression in gastric cancer Journal of experimental & clinical cancer research : CR 2022-07-12 [PMID: 35820928] (In vitro, Human) | In vitro | Human |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | WWTR1 |
![SDS-Page: Recombinant Human TAZ/WWTR1 Protein [NBP2-22949]](http://images.novusbio.com/fullsize/TAZ-Protein-SDS-Page-NBP2-22949-img0001.jpg "SDS-Page: Recombinant Human TAZ/WWTR1 Protein [NBP2-22949]")
![Immunocytochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]](https://images.novusbio.com/images2/RUNX2_MAB2006_Immunocytochemistry_13309.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9968.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9969.jpg)
![Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg)
![FGF basic/FGF2/bFGF [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "FGF basic/FGF2/bFGF [Unconjugated]")
![Immunoprecipitation: TAZ/WWTR1 Antibody - BSA Free [NB110-58359] - TAZ knockout in muscle decreases mitochondrial mass, respiration, & exercise ability. b Proteins of gastrocnemius muscle of WT & mKO mice analysed via immunoblotting for detection of mitochondrial marker proteins. Alpha-tubulin used as loading control. Representative data shown & experiment performed twice w/ similar results. Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/35115527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](https://images.novusbio.com/images/nb110-58359_rabbit-polyclonal-taz-wwtr1-antibody-3102024153977.jpg "Immunoprecipitation: TAZ/WWTR1 Antibody - BSA Free [NB110-58359] - TAZ knockout in muscle decreases mitochondrial mass, respiration, & exercise ability. b Proteins of gastrocnemius muscle of WT & mKO mice analysed via immunoblotting for detection of mitochondrial marker proteins. Alpha-tubulin used as loading control. Representative data shown & experiment performed twice w/ similar results. Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/35115527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")