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TAZ/WWTR1 Antibody (CL0371)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining in human placenta and lymph node tissues. Corresponding WWTR1 RNA-seq data are presented for the same ...read more
Genetic Strategies: Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-WWTR1 antibody. Remaining ...read more
Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining in SKMEL cell line with Anti-WWTR1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are ...read more
Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human placenta shows strong nuclear and some cytoplasmic positivity in endothelial cells.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules and in glomeruli.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human lymph node shows no nuclear positivity in lymphoid cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
CL0371
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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TAZ/WWTR1 Antibody (CL0371) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: MNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSST
Epitope
SSWRKKILPESFFKE
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
WWTR1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TAZ/WWTR1 Recombinant Protein Antigen (NBP2-52926PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for TAZ/WWTR1 Antibody (CL0371)

  • FLJ27004
  • FLJ45718
  • TAZ
  • TAZDKFZp586I1419
  • transcriptional co-activator with PDZ-binding motif
  • Transcriptional coactivator with PDZ-binding motif
  • WW domain containing transcription regulator 1
  • WW domain-containing transcription regulator protein 1
  • WWTR1

Background

TAZ is a YAP-related protein that contains a WW domain and transcriptional activation region. TAZ plays a role in mesenchymal stem cell differentiation. The antibody was designed in such a way that should generate a TAZ specific-reagent able to differentiate between YAP and TAZ proteins in cells and tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TAZ/WWTR1 Antibody (NBP2-52926). (Showing 1 - 1 of 1 FAQ).

  1. I want to know which TAZ primary antibody is best produced for WB (human and mouse)!
    • I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.
mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol WWTR1