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Syntaxin-BP1 Antibody - BSA Free

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Western Blot: Syntaxin-BP1 Antibody [NB120-3451] - Analysis of rat brain homogenate immunoprecipitated. 1. Extract only. 2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of PA1-742, respectively.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Syntaxin-BP1 Antibody - BSA Free Summary

Immunogen
Synthetic Peptide: V(58) E D I N K R R E P I P S(70)
Specificity
Munc 18
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STXBP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 67 kDa protein representing Munc-18 from rat whole brain extract.

Reactivity Notes

Expected cross-reactivity with Bovine and Canine base on sequence identity.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Syntaxin-BP1 Antibody - BSA Free

  • EIEE4
  • FLJ37475
  • hUNC18
  • MUNC18-1
  • NSEC1
  • N-Sec1
  • p67
  • Protein unc-18 homolog 1
  • Protein unc-18 homolog A
  • rbSec1
  • STXBP1
  • syntaxin binding protein 1
  • syntaxin-binding protein 1
  • SyntaxinBP1
  • Syntaxin-BP1
  • UNC18
  • unc18-1
  • UNC18A
  • unc-18A
  • UNC18neuronal SEC1

Background

Munc-18 is a cytosolic brain protein of ~67 kDa which plays a key role in vesicle membrane fusion events with the plasma membrane. Functional interactions have been demonstrated to exist between Munc-18, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin. Munc-18 is the mammalian homologue of the C. elegans gene unc-18. Mutations in Munc-18 can result in accumulations of acetylcholine and paralytic phenotype suggestive of a neurotransmitter release defect. Munc-18 thus appears to be required by the synaptic vesicle fusion complex. Two Munc-18 isoforms have recently been identified. Munc-18-1 has been shown to be primarily expressed in brain, while Munc-18-2 is expressed in most tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STXBP1
Entrez