![Western Blot: SUB1 Antibody (04) [NBP3-06541] - Lane A: NIH-3T3 Whole Cell LysateLane B: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:16 kDa](http://images.novusbio.com/fullsize/SUB1-Antibody-04-Western-Blot-NBP3-06541-img0002.jpg "Western Blot: SUB1 Antibody (04) [NBP3-06541] - Lane A: NIH-3T3 Whole Cell LysateLane B: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:16 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clone | 04 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SUB1 (Uniprot#: P53999; Met1-Leu127). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SUB1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SUB1 Antibody (NBP3-06541)Find related products by research area.
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| Gene Symbol | SUB1 |
![Immunocytochemistry/Immunofluorescence: SUB1 Antibody (04) [NBP3-06541] - Staining of Human SUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SUB1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody(red) and counterstained with DAPI(blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/SUB1-Antibody-04-Immunocytochemistry-Immunofluorescence-NBP3-06541-img0003.jpg "Immunocytochemistry/Immunofluorescence: SUB1 Antibody (04) [NBP3-06541] - Staining of Human SUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SUB1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody(red) and counterstained with DAPI(blue). Positive staining was localized to nucleus.")
![Immunoprecipitation: SUB1 Antibody (04) [NBP3-06541] - Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-SUB1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SUB1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa](http://images.novusbio.com/fullsize/SUB1-Antibody-04-Immunoprecipitation-NBP3-06541-img0001.jpg "Immunoprecipitation: SUB1 Antibody (04) [NBP3-06541] - Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-SUB1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SUB1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")