Novus Biologicals products are now on bio-techne.com

STI1 Antibody (1E3)

Images

 
Western Blot: STI1 Antibody (1E3) [H00010963-M11] - Analysis of STIP1 expression in Raw 264.7 (Cat # L024V1).
Immunocytochemistry/ Immunofluorescence: STI1 Antibody (1E3) [H00010963-M11] - Analysis of monoclonal antibody to STIP1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: STI1 Antibody (1E3) [H00010963-M11] - Analysis of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml
Western Blot: STI1 Antibody (1E3) [H00010963-M11] - Analysis of STIP1 expression in HeLa (Cat # L013V1).
Western Blot: STI1 Antibody (1E3) [H00010963-M11] - Analysis of STIP1 expression in PC-12 (Cat # L012V1).
Western Blot: STI1 Antibody (1E3) [H00010963-M11] - Analysis of STIP1 expression in NIH/3T3 (Cat # L018V1).
ELISA: STI1 Antibody (1E3) [H00010963-M11] - Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

STI1 Antibody (1E3) Summary

Immunogen
STIP1 (NP_006810.1, 445 a.a. ~ 543 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR
Specificity
STIP1 (1E3)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STIP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using H00010963-M11.

Reactivity Notes

Human, Mouse & Rat. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STI1 Antibody (1E3)

  • HOP
  • HOPSTI1L
  • Hsc70/Hsp90-organizing protein
  • Hsp70/Hsp90-organizing protein
  • IEF-SSP-3521
  • NY-REN-11 antigen
  • NY-REN-11
  • Renal carcinoma antigen NY-REN-11
  • STI1
  • STI1L
  • STI1P60
  • STIP1
  • stress-induced-phosphoprotein 1
  • Transformation-sensitive protein IEF SSP 3521

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 (PubMed 16100115)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-81696
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB120-2928
Species: Hu, Mu, Pm, Rb, Rt, Sh
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-90267
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NB300-576
Species: Ch, Gp, Hu, Mu, Pm, Rb, Rt, Xp
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP3-13279
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00007178-M06
Species: Hu, Mu, Rt
Applications: ELISA, WB
NBP1-82454
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB110-57586
Species: Bv, Ch, Hu, Mu, Pm, Rb, Rt
Applications: IHC, IHC-P, KD, WB
NB100-56161
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
AF771
Species: Mu
Applications: IHC, Neut, WB
NBP1-81404
Species: Hu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP2-15939
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-03433
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB300-731
Species: Gp, Hu, Mu, Rb, Rt, Sh, Ye
Applications: B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB100-56605
Species: Av, Bv, Sh
Applications: WB
NB120-2788
Species: Bv, Fe, Fi, Ha, Hu, Mu, Pm, Rt
Applications: B/N, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB

Publications for STI1 Antibody (H00010963-M11)(2)

Reviews for STI1 Antibody (H00010963-M11) (0)

There are no reviews for STI1 Antibody (H00010963-M11). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STI1 Antibody (H00010963-M11) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional STI1 Products

Research Areas for STI1 Antibody (H00010963-M11)

Find related products by research area.

Blogs on STI1.

Chaperone Mediated Autophagy (CMA) does it all!
By Christina Towers, PhD. The degradation of cellular proteins is a critical step of both regulation and quality control and results in the turn over and recycling of critical amino acids. The two main mechanisms o...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our STI1 Antibody (1E3) and receive a gift card or discount.

Bioinformatics

Gene Symbol STIP1
Entrez
OMIM
Uniprot