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ST2/IL-33R Antibody (245714) [Unconjugated]

 (2 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
245714
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB1004-SP
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ST2/IL-33R Antibody (245714) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
Ser27-Arg332
Accession # P14719
Specificity
Detects mouse ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human ST2 and no cross-reactivity with recombinant  mouse (rm) IL-1 R1 or rmIL-1 R2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Il1rl1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1004 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST2/IL-33R Antibody (245714) [Unconjugated]

  • DER4
  • DER4ST2growth stimulation-expressed
  • Fit-1
  • IL-1 R4
  • IL1R4
  • IL-1R4
  • IL-1RL1
  • IL-33 R
  • IL33R
  • IL-33R
  • interleukin 1 receptor-like 1
  • interleukin 1 receptor-related protein
  • interleukin-1 receptor-like 1
  • Ly84
  • MGC32623
  • Protein ST2
  • ST2
  • ST2L
  • ST2V
  • T1
  • T1homolog of mouse growth stimulation-expressed

Background

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4‑7). It binds IL-33, a cytokine that is up‑regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11‑13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33's ability to signal through transmembrane ST2 (9, 12‑14).
  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
  4. Schmitz, J. et al. (2005) Immunity 23:479.
  5. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  6. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  7. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  8. Sanada S. et al. (2007) J. Clin. Invest. 117:1538
  9. Palmer, G. et al. (2008) Cytokine 42:358.
  10. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  11. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  12. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  13. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  14. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ST2/IL-33R Antibody (MAB1004)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


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(1)
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Bioinformatics

Gene Symbol Il1rl1
Uniprot