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SSEA-1 Antibody (FR4A5)

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Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (FR4A5) [NBP2-45304] - ICC/IF staining of U937 cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (FR4A5) [NBP2-45304] - ICC/IF staining of U937 cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Flow Cytometry: SSEA-1 Antibody (FR4A5) [NBP2-45304] - Flow Cytometric Analysis of U937 cells using SSEA-1 Antibody (FR4A5) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, ICC/IF
Clone
FR4A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-11605
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SSEA-1 Antibody (FR4A5) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11605)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Myelomonocytic leukemia cells
Localization
Cell surface and granular paranuclear
Marker
Reed-Sternberg Cell Marker
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FUT4
Purity
Protein L or PEG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein L or PEG purified

Alternate Names for SSEA-1 Antibody (FR4A5)

  • alpha-(1,3)-fucosyltransferase
  • CD15
  • EC 2.4.1
  • EC 2.4.1.-
  • EC 2.4.1.65
  • ELAM-1 ligand fucosyltransferase
  • ELFT
  • FCT3AELAM ligand fucosyltransferase
  • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
  • Fucosyltransferase 4
  • Fucosyltransferase IV
  • FUC-TIV
  • fucT-IV
  • FUTIV
  • Galactoside 3-L-fucosyltransferase
  • Lewis X
  • LeX
  • SSEA1
  • SSEA-1
  • stage-specific embryonic antigen 1

Background

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FUT4
Uniprot