Novus Biologicals products are now on bio-techne.com

SPG21 Recombinant Protein Antigen

Images

 
There are currently no images for SPG21 Recombinant Protein Antigen (NBP2-56164PEP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

SPG21 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to SPG21.

Source: E. coli

Amino Acid Sequence: AKEEMYKLYPNARRAHLKTGGNFPYLCRSAEVNLYVQIHLLQFHGTKYAAIDPSMVSAEELEVQKGSLGISQEEQ

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
SPG21
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-56164.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for SPG21 Recombinant Protein Antigen

  • Acid cluster protein 33
  • ACP33Spastic paraplegia 21 protein
  • GL010
  • MASPARDIN
  • MASTBM-019
  • spastic paraplegia 21 (autosomal recessive, Mast syndrome)
  • Spastic paraplegia 21 autosomal recessive Mast syndrome protein

Background

SPG21 is encoded by this gene was identified by a two-hybrid screen using CD4 as the bait. It binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation. The interaction with CD4 is mediated by the noncatalytic alpha/beta hydrolase fold domain of this protein. It is thus proposed that this gene product modulates the stimulatory activity of CD4. At least three different transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00168400-D01P
Species: Hu
Applications: WB
NBP1-88502
Species: Hu
Applications: IHC, IHC-P
1090-TW/CF
Species: Hu
Applications: BA
NBP1-76990
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
DY413
Species: Mu
Applications: ELISA
NBL1-14964
Species: Hu
Applications: WB
NBP2-01650
Species: Hu, Pm, Mu
Applications: Flow, IHC, IHC-P, WB
NBP1-86644
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-76393
Species: Hu, Mu
Applications: CHIP-SEQ, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-81854
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP1-86641
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
H00002678-M01
Species: Hu, Mu, Ye
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP1-86616
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
2914-HT
Species: Hu
Applications: BA
NBP2-01860
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-47914
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-78039
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow-CS, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-56164PEP
Species: Hu
Applications: AC

Publications for SPG21 Recombinant Protein Antigen (NBP2-56164PEP) (0)

There are no publications for SPG21 Recombinant Protein Antigen (NBP2-56164PEP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SPG21 Recombinant Protein Antigen (NBP2-56164PEP) (0)

There are no reviews for SPG21 Recombinant Protein Antigen (NBP2-56164PEP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for SPG21 Recombinant Protein Antigen (NBP2-56164PEP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SPG21 Products

Research Areas for SPG21 Recombinant Protein Antigen (NBP2-56164PEP)

Find related products by research area.

Blogs on SPG21

There are no specific blogs for SPG21, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)

As an example, if the molecular weight of a compound is 197.13 g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = 19.713 (value determined by this calculator).

=
x
x
g/mol

Review this Product

Be the first to review our SPG21 Recombinant Protein Antigen and receive a gift card or discount.

Bioinformatics

Gene Symbol SPG21