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Recombinant Human Soluble Liver/Pancreas Antigen His Protein

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SDS-Page: Recombinant Human Soluble Liver/Pancreas Antigen His Protein [NBP1-50890] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

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Recombinant Human Soluble Liver/Pancreas Antigen His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-501 of Human Soluble Liver/Pancreas Antigen

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SEPSECS
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
57.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.5), 40% glycerol, 0.15 M NaCl, 1 mM DTT
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Soluble Liver/Pancreas Antigen His Protein

  • EC 2.9.1.2
  • EC 2.9.1.n1
  • Liver-pancreas antigen
  • LPDKFZp434B1417
  • MGC161491
  • O-phosphoseryl-tRNA(Sec) selenium transferase
  • Sec synthase
  • Selenocysteine synthase
  • Selenocysteinyl-tRNA(Sec) synthase
  • Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
  • SepSecS
  • Sep-tRNA:Sec-tRNA synthase
  • SLA/LP autoantigen
  • SLA/LP
  • SLA-p35
  • SLAUGA suppressor tRNA-associated protein
  • Soluble liver antigen
  • soluble liver antigen/liver pancreas antigen
  • tRNA(Ser/Sec)-associated antigenic protein
  • TRNP48

Background

SEPSECS, also known as SLA/LP autoantigen, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis. Recombinant human SEPSECS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SEPSECS