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Sodium Potassium ATPase Alpha 1 Antibody (464.6) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Dr, Gp, Pm, Rb, Sh, Xp, YeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
464.6
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

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Sodium Potassium ATPase Alpha 1 Antibody (464.6) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla. [UniProt# Q9N0Z6]
Specificity
This is specific for Na,K-ATPase alpha 1 subunit.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ATP1A1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Sodium Potassium ATPase Alpha 1 Antibody (464.6) [CoraFluor™ 1]

  • ATP1A1
  • ATPase, Na+/K+ transporting, alpha 1 polypeptide
  • EC 3.6.3
  • EC 3.6.3.9
  • EC 7.2.2.13
  • K-ATPase alpha-1 subunit
  • K-ATPase catalytic subunit alpha-A protein
  • MGC3285
  • MGC51750
  • Na
  • Na(+)/K(+) ATPase alpha-1 subunit
  • Na, K-ATPase, alpha-A catalytic polypeptide
  • Na+, K+ ATPase alpha subunit
  • Na+/K+ ATPase 1
  • Sodium Potassium ATPase Alpha 1
  • sodium pump 1
  • Sodium pump subunit alpha-1
  • sodium/potassium-transporting ATPase subunit alpha-1
  • sodium-potassium-ATPase, alpha 1 polypeptide

Background

Sodium Potassium ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The Sodium Potassium ATPase enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide (Sodium Potassium ATPase Alpha 1) has been shown to be the catalytically active subunit, whereas the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Sodium Potassium ATPase alpha-1 subunit, in the brain, is expressed in both neuronal and non-neuronal cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sodium Potassium ATPase Alpha 1 Antibody (NBP2-61137CL1). (Showing 1 - 1 of 1 FAQ).

  1. I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.
    • Our sodium potassium ATPase and plasma membrane products may be of use to you. Please contact us at technical@novusbio.com with any additional questions.

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Bioinformatics

Gene Symbol ATP1A1