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SNF5 Antibody (CL14031)

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Staining of human lymph node shows strong nuclear positivity in non-germinal and germinal center cells.
Staining of human lymph node shows strong nuclear positivity in non-germinal and germinal center cells.
Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.
Staining in RH-30 cell line with Anti-SMARCB1 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Western blot analysis in human cell line SK-MEL-30.
Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.
Staining of human prostate shows strong nuclear positivity in glandular cells.
Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Staining of human endometrial cancer shows strong nuclear positivity in tumor cells.
Staining of human colon shows strong nuclear positivity in glandular cells and lymphoid cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL14031
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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SNF5 Antibody (CL14031) Summary

Immunogen
This antibody was designed to target the recombinant protein SNF5, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SMARCB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000-1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for SNF5 Antibody (CL14031)

  • BAF47
  • BAF47hSNF5
  • BRG1-associated factor 47
  • hSNFS
  • Ini1
  • INI1RTPS1
  • Integrase interactor 1 protein
  • malignant rhabdoid tumor suppressor
  • RDTSNF5 homolog
  • Sfh1p
  • SNF5
  • SNF5L1
  • Snr1
  • subfamily b, member 1
  • sucrose nonfermenting, yeast, homolog-like 1
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily B member 1

Background

SNF5 is encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SNF5 Antibody
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NBP3-24595
SNF5 Antibody (CL14031)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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You can't be without me - SNF5
The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a ...  Read full blog post.
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Bioinformatics

Gene Symbol SMARCB1