![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HepG2.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0017.jpg "Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HepG2.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clone | 1D7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | SMURF1 (NP_065162, 165 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIP |
| Specificity | SMURF1 (1D7) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SMURF1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SMURF1 Antibody (H00057154-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Immunocytochemistry-Immunofluorescence-H00057154-M01-img0011.jpg "Immunocytochemistry/Immunofluorescence: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Immunohistochemistry-Paraffin-H00057154-M01-img0013.jpg "Immunohistochemistry-Paraffin: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 2 ug/ml.")
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 expression in Raw 264.7 ( Cat # L024V1 ).](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0002.jpg "Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 expression in Raw 264.7 ( Cat # L024V1 ).")
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0003.jpg "Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 expression in NIH/3T3 ( Cat # L018V1 ).")
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HeLa.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0016.jpg "Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HeLa.")
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody (M01), clone 1D7.Lane 1: SMURF1 transfected lysate(83.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0018.jpg "Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody (M01), clone 1D7.Lane 1: SMURF1 transfected lysate(83.4 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SMURF1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Immunoprecipitation-H00057154-M01-img0014.jpg "Immunoprecipitation: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SMURF1 MaxPab rabbit polyclonal antibody.")
![ELISA: SMURF1 Antibody (1D7) [H00057154-M01] - Detection limit for recombinant GST tagged SMURF1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-ELISA-H00057154-M01-img0019.jpg "ELISA: SMURF1 Antibody (1D7) [H00057154-M01] - Detection limit for recombinant GST tagged SMURF1 is approximately 0.1ng/ml as a capture antibody.")
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