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Human SMPD1 ELISA Kit (Colorimetric)

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ELISA: Human SMPD1 ELISA Kit (Colorimetric) [NBP2-69873] - Samples were spiked with high concentrations of Human SMPD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human SMPD1 ELISA Kit (Colorimetric) [NBP2-69873] - Samples were spiked with high concentrations of Human SMPD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human SMPD1 ELISA Kit (Colorimetric) [NBP2-69873] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Human SMPD1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human ASM in samples. No significant cross-reactivity or interference between Human ASM and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.18%
Intra-Assay
CV% < 5%
Spike Recovery
92-112%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SMPD1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human SMPD1 ELISA Kit (Colorimetric)

  • Acid sphingomyelinase
  • aSMase
  • ASMsphingomyelin phosphodiesterase
  • EC 3.1.4.12
  • NPD
  • SMPD1
  • sphingomyelin phosphodiesterase 1, acid lysosomal

Background

The protein encoded by the SMPD1 gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Three transcript variants encoding two different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human SMPD1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol SMPD1