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SMG1 Antibody (1G5)

Images

 
Immunocytochemistry/ Immunofluorescence: SMG1 Antibody (1G5) [H00023049-M01] - Analysis of monoclonal antibody to SMG1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: SMG1 Antibody (1G5) [H00023049-M01] - Detection limit for recombinant GST tagged SMG1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SMG1 Antibody (1G5) Summary

Immunogen
SMG1 (NP_055535, 2922 a.a. ~ 3031 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV
Specificity
SMG1 - PI-3-kinase-related kinase SMG-1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SMG1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It is also useful for immunofluorescence.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SMG1 Antibody (1G5)

  • ATXserine/threonine-protein kinase SMG1
  • EC 2.7.11
  • EC 2.7.11.1
  • hSMG-1,61E3.4
  • KIAA0421phosphatidylinositol 3-kinase-related protein kinase
  • Lambda/iota protein kinase C-interacting protein
  • Lambda-interacting protein
  • LIPPI-3-kinase-related kinase SMG-1
  • phosphatidylinositol 3-kinase-related kinase
  • SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
  • SMG-1

Background

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional SMG1 Products

Array H00023049-M01

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Bioinformatics

Gene Symbol SMG1
Entrez
OMIM
Uniprot