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SMARCC1 Antibody

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Western Blot: SMARCC1 Antibody [NBP1-88721] - Analysis in human cell line NTERA-2.
Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody [NBP1-88721] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry: SMARCC1 Antibody [NBP1-88721] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.
Immunohistochemistry: SMARCC1 Antibody [NBP1-88721] - Staining of human placenta shows distinct nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP1-88721] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP1-88721] - Staining of human testis shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP1-88721] - Staining in human testis and skeletal muscle tissues using anti-SMARCC1 antibody. Corresponding SMARCC1 RNA-seq data are ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP1-88721] - Analysis in human testis and skeletal muscle tissues. Corresponding SMARCC1 RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP1-88721] - Staining of human placenta, skeletal muscle, testis and tonsil using Anti-SMARCC1 antibody NBP1-88721 (A) shows similar ...read more
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP1-88721] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP1-88721] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SMARCC1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AHVKKVQEAARASGKVDPTYGLESSCIAGTGPDEPEKLEGAEEEKMEADPDGQQPEKAENKVENETDEGDKAQDGENEKNSEKEQDSEVSEDTKSEEKETEENKELTDTCKERESDTGKKKVEHEISEGNVATAAAAALASAATKAKHL
Predicted Species
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SMARCC1 Protein (NBP1-88721PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SMARCC1 Antibody

  • BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1
  • BRG1-associated factor 155
  • chromatin remodeling complex BAF155 subunit
  • CRACC1
  • mammalian chromatin remodeling complex BRG1-associated factor 155
  • Rsc8
  • SRG3
  • subfamily c, member 1
  • SWI/SNF complex 155 kDa subunit
  • SWI/SNF complex subunit SMARCC1
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI3

Background

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCC1/BAF155 is part of the BAF complex that associates with SMARCA2/Brm and SMARCA4/Brg. The mouse homolog is Srg3 and has been found to be required for embryogenesis and brain development. SMARCC1 is also identified as SWI/SNF complex 155 kDa subunit, and BRG1-associated factor 155.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMARCC1